| The Efficacy of alpha-lipoic Acid on the Endotoxin-induced Acute Lung Injury. |
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Jin Won Huh, Sang Bum Hong, Mi Jung Kim, Chae Man Lim, Younsuck Koh |
1Department of Internal Medicine, Ilsan Paik Hospital, Inje University, Goyang, Korea,
2Division of Pulmonary and Critical Care Medicine, Asan Medical Center, University of ulsan College of Medicine, Seoul, Korea. yskoh@amc.seoul.kr
3Division of Pulmonary and Critical Care Medicine, Asan Medical Center, Asan Institute for Life Sciences, Seoul, Korea. |
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| Abstract |
BACKGROUND
Oxidative stress may play an important role in the pathogenesis of endotoxin-induced acute lung injury (ALI). This study evaluated the therapeutic effect of alpha-lipoic acid, a nonenzymatic antioxidant, in a rat model of lipopolysaccharide (LPS) induced ALI. MATERIALS AND METHODS: ALI was induced in Sprague-Dawley rats by instilling LPS (E.coli, 3mg/Kg) into the trachea. The rats were classified into the control, control+alpha-lipoic acid, LPS, and LPS+alpha-lipoic acid groups.The lung lavage neutrophil count, cytokine-induced neutrophil chemoattractant (CINC), lung myeloperoxidase (MPO), and cytokine concentrations (TNF-alpha, IL-1beta, IL-6 and IL-10) were measured at 2 h and 6 h after LPS administration. RESULTS: The total cell and neutrophil counts of the LPS+alpha-lipoic acid groups were significantly lower than the LPS groups. The protein concentration in the BAL fluid was similar in the LPS groups and LPS+alpha-lipoic acid groups. The TNF-alpha, IL-1beta, and IL-6 concentrations in the BAL fluid were not decreased by the alpha-lipoic acid treatment in the LPS treated rats. CONCLUSIONS: Although alpha-lipoic acid decreased the level of LPS-induced neutrophil infiltration into the lung, it could not attenuate the LPS-induced ALI at the dose administered in this study. |
| Key Words:
alpha-lipoic acid, oxidative stress, acute lung injury, antioxidant |
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