| Construction of Recombinant BCGs Overexpressing Antigen 85 Complex and Their Protective Efficacy against Mycobacterium tuberculosis Infection in a Mouse Model. |
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Seung Heon Lee, Bo Young Jeon, Young Gil Park, Hye Young Lee, Sang Nae Cho, Hyo Joon Kim, Gill Han Bai |
1Department of Molecular Biology, Korean Institute of Tuberculosis, Seoul, Korea. gbai@hotmail.com
2Department of Microbiology, Yonsei University College of Medicine, Seoul, Korea.
3Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Korea.
4Department of Biochemistry and Molecular Biology, College of Science Technology, Hanyang University, Ansan, Korea. |
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| Abstract |
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Tuberculosis (TB) remains an enormous global health problem, and a new vaccine against TB more potent than the current inadequate BCG vaccine is urgently needed. We constructed three recombinant Mycobacterium bovis BCG (rBCG) strains over-expressing antigen (Ag) 85A, Ag85B, or both of M. tuberculosis using their own promoter and secretory sequence, or hsp60 promoter. SDS-PAGE analysis of rBCG proteins showed over-expression of Ag85A and Ag85B proteins in higher level than of those in their parental strain of BCG. In addition, rBCG(rBCG/B.FA) over-expressing Ag85A and Ag85B induced strong IFN-gamma production in splenocytes. However, there was no significant difference in protective efficacy between rBCG and their parental BCG strain. In this study, therefore, rBCG over-expressing Ag85A, Ag85B, or both failed to show enhanced protection against M. tuberculosis infection in a mouse model. |
| Key Words:
Tuberculosis, Vaccine, Recombinant BCG |
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