Gene Expression of Surfactant Protein A mRNA of the Lung in Endotoxin and Thiourea Treated Rats.

Lee, Jae Young; Kim, Mi Ok; Sohn, Jang Won; Yoon, Ho Joo; Shin, Dong Ho; Kim, Tae Wha; Park, Sung Soo

doi:2003.55.3.257

Tuberc Respir Dis > Volume 55(3); 2003 > Article

Original Article

Tuberculosis and Respiratory Diseases 2003;55(3):257-266.
DOI: https://doi.org/10.4046/trd.2003.55.3.257 Published online September 1, 2003.

Gene Expression of Surfactant Protein A mRNA of the Lung in Endotoxin and Thiourea Treated Rats.

Jae Young Lee, Mi Ok Kim, Jang Won Sohn, Ho Joo Yoon, Dong Ho Shin, Tae Wha Kim, Sung Soo Park

¹Department of Internal Medicine, Hanyang University, College of Medicine, Seoul, Korea. parkss@hanyang.ac.kr
²Department of Internal Medicine, Hallym University, College of Medicine, Seoul, Korea.

Abstract

BACKGROUND
The surfactant protein A(SP-A) is important in the regulation of surfactant secretion, synthesis and recycling. Since the acute respiratory distress syndrome(ARDS) is usually viewed as the functional and morphological expression of a similar underlying lung injury caused by a variety of insults and since abnormalities in surfactant function have been described in ARDS, the authors investigated the different effects of endotoxin and thiourea on the accumulation of mRNA encoding SP-A. METHODS: Sprague-Dawley rats were given 5 mg/kg intraperitoneal endotoxin from Salmonella enteritidis and 3.5 mg/kg intraperitoneal thiourea and sacrified at different time periods. RESULTS: 1) SP-A mRNA was significantly increased 67.0% in 6 hours and 73.4% in 24 hours after 5 mg/kg endotoxin treatment respectively(P<0.005, P<0.005). 2)SP-A mRNA was significantly decreased 32.9% in 24 hours after 3.5 mg/kg thiourea treatment(P<0.05). CONCLUSIONS: These results indicate that the differential regulation of surfactant protein A in vivo is evident and suggest that surfactant protein A might be differentially regulated during different kind of insults of lung injury at different time periods without altering lung wet to dry ratios.

Key Words: Gene expression, Surfactant protein A, Endotoxin & Thiourea