Adherence-induced gene expression in human alveolar macrophages.

Chung, Man Pyo; Yoo, Chul Gyu; Han, Sung Koo; Shim, Young Soo; Rhee, Chong H.; Han, Yong Chol; Kim, Young Whan

doi:1996.43.6.936

Tuberc Respir Dis > Volume 43(6); 1996 > Article

Original Article

Tuberculosis and Respiratory Diseases 1996;43(6):936-944.
DOI: https://doi.org/10.4046/trd.1996.43.6.936 Published online December 1, 1996.

Adherence-induced gene expression in human alveolar macrophages.

Man Pyo Chung, Chul Gyu Yoo, Sung Koo Han, Young Soo Shim, Chong H. Rhee, Yong Chol Han, Young Whan Kim

¹Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.
²Tuberculosis Research Institute, Seoul National University College of Medicine, Seoul, Korea.

Abstract

Background
Neutrophils or monocytes separated in vitro by the adherence to plastic surface are known to be activated by surface adherence itself and subsequent experimental data might be altered by surface adherence. Adhesion molecules and gene transcription of the inflammatory mediators are known to be associated in this process. To evaluate whether adhesion molecule and transcriptional activation of the inflammatory substances are also involved in the activation of human alveolar macrophage by the adherence procedure, we designed this experiment. Method: Bronchoalveolar lavage was performed in the person whose lung of either side was confirmed to be normal by chest CT and alveolar macrophage was harvested. To measure the expression of Interleukin-8(IL-8) mRNA, manganese superoxide dismutase(SOD) mRNA and CD11/CD18 mRNA in human alveolar macrophage of both adherence state and suspension state, Northern blot analysis was done at 0, 2, 4, 8 and 24hrs after the adherence to plastic surface and during suspension state. Then, phorbol myristate acetate(PMA) and N-formyl-methionyl-leucyl- phenylalanine(fMLP) were added respectively in the same experimental condition. Result: 1) Human alveolar macrophages in the adherent state induced IL-8 mRNA and SOD mRNA expression which was maximal at 8 hours after the adherence to plastic surface. But we could not observe the upregulation of CD18 mRNA by surface adherence. 2) PMA induced these mRNA expression both in the adherent cell and the nonadherent cells, but the induction of mRNA expression by fMLP occurred only in the adherent cells. Conclusion: These results suggest that adherence of huamn alveolar macropahge is an important cell-activating event that may play a critical role in the modulation of lung inflammatory response.

Key Words: Alveolar macrophage, Adherence, Interleukin-8