| Enzyme-Linked Immunosorbent Assay (ELISA) for the Detection of Antibody to Mycobacterium tuberculosis in Sera from Patients with Pulmonary Tuberculosis |
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Sang Jae Kim, Gill Han Bai, Sung Chin Kim |
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Korean Institute of Tuberculosis, Korean National Tuberculosis Association, Seoul, Korea |
| 효소 결합 면역 분석법을 이용한 결핵환자 혈청 내 항결핵균항체의 검출 |
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김상재, 배길한, 김성진 |
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| Abstract |
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The leveIs of IgG antibody binding to the soIuble antigens of Mycobacterium boνis BCG in sera from patients with tuberculosis and healthy individuals in Korea were measured by the enzyme-Iinked immunosorbent assay (ELISA). The most reactive antigenic components with homologous goat hyperimmune serum distributed in the fraction, which was extracted from Iive BCG cells homogenized by French-press at 15, 000 psi and was precipitable at 75% saturation of ammonium sulfate.
Thirty eight (79.2) out of 48 sputum positive patients and 23 (45.1 %) out of 51 sputum negative but radiologicaIIy suspected cases showed a positive reaction at the serum diIution 1 : 400, at which none of 24 healthy individuals showed a positive reaction. At a serum diIution 1: 3, 200, 29.2% of sputum positive patients showed a positive reaction whiIe 11.8% of sputum negative cases showed a positive reaction. Of 13 smear negative culture positive cases, 10 were positive at the serum diIution 1 : 400. Fifteen of 24 heaIthy individuals were positive at the serum diIution 1 : 50, 6 at 1 : 100, and 1 at 1 : 200. |
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