Autoradiographic Labeling Index of Tritiated Thymidine in Human Lung Cancer

Park, Sung Soo; Lee, Seong Jun; Lee, Dong Hoo; Lee, Jung Hee; Lee, Jung Dal

doi:1989.36.2.134

Tuberc Respir Dis > Volume 36(2); 1989 > Article

Original Article

Tuberculosis and Respiratory Diseases 1989;36(2):134-141.
DOI: https://doi.org/10.4046/trd.1989.36.2.134 Published online June 1, 1989.

Autoradiographic Labeling Index of Tritiated Thymidine in Human Lung Cancer

Sung Soo Park¹, Seong Jun Lee¹, Dong Hoo Lee¹, Jung Hee Lee¹, Jung Dal Lee²

¹Departments of Internal Medicine, School of Medicine, Hanyang University, Seoul, Korea
²Departments of Pathology, School of Medicine, Hanyang University, Seoul, Korea

인체 폐암에서의 Autoradiography 에 의한 3H-Thymidine Labeling Index에 관한 연구

박성수¹, 이성준¹, 이동후¹, 이정희¹, 이중달²

Abstract

For the assessment of tumor cel1 proliferation, in vitro thymidine labeling indices of human lung cancers and squamous metaplasia were measured employing autoradiography of paraffin-embedded tissue sections. The tissues for culture were obtained with fiberoptic bronchoscopic biopsy from the 19 patients. The biopsy specimens consisted of eight squamous cell carcinomas, five small cell carcinomas, and six squamous metaplasias, The biopsy specimens were incubated with Eagle’s MEM culture media containing tritiated thymidine (specific activity, 20 Ci/mmol) for 30 minutes. The mean value with standard deviation of thymidine labeling indices was 20.54 ± 5.57% for squamous cell carcinomas, 19.20 ± 5.19% for small cell carcinomas and 7.94 ± 2.29% for squamous metaplasias, respectively. A wide range of individual labeling indices was noticed in the lung cancers In conclusion, tritiated thymidine labeling indices of human lung cancers were significantly higher than those of squamous metaplasia that may probably be an preneoplastic lesion.