Tuberc Respir Dis > Volume 39(2); 1992 > Article
Tuberculosis and Respiratory Diseases 1992;39(2):131-140.
DOI:    Published online April 1, 1992.
Histopathological changes in rat lung instilled with natural coal and free silica dust.
Kyung Ah Kim1, Chang Young Jung1, Sang Young Oh1, Hyeon Woo Yim1, Young Lim1, Im Goung Yun1, Young Man Roh2
1Department of Occupational Disease, Catholic University Medical College, Seoul, Korea
2Catholic lndustrial Medical Center, Catholic University Medical College, Seoul, Korea
ln order to investigate the changes of hydroxyproline amount and pathologic finding in rat lung which were instilled the natural coal and free silica dust intratracheally, the subjects were divided into two groups as follows. The control group was only administered intratracheally 0.5 ml of normal saline, and the experimental groups were instilled at once the turbid solution containing 10 mg, 30 mg and 50 mg of natural coal and free silica dust each, subjects were sacrified at the 3rd and the 20th week each after the experiment. Hydroxyproline amount in the right upper lung was measured by Woessner method and HPLC (modified Dunphy) method, and the pathlogic finding of lung tissue were observed for hematoxylin-eosin staining, Bielschowski method and trichrome method. The results were as follows . 1) The wet lung weights of all experimental groups excluding in the groups instilled 10 mg and 30 mg of natural coal dust at 3rd week , were significantly increased (p < 0.05) compared with control group. The weight in each free silica group was markedly increased (p < 0.05) at 20th week compared with the same dose of natural coal dust group, while the weight in the same dose group of free silica dust was increased significantly at 20th week compared with at 3rd week. 2) The amount of hydroxyproline were significantly increased (p < 0.05) in the natural coal and free silica groups at 20th week compared with the control groups, and in each experimental group instilled the same kind and dose of dust , its amount was markedly increased (p < 0.05) at 20th week compared with at 3rd week. And also the hydroxyproline in 30 mg and 50 mg free silica groups increased markedly (p < 0.05) at 20th week compared with the natural coal dust of the same dose 3) The polymorphonuclear leukocytes, fibroblasts and macrophages in interstitium and alveolar space showed the increasing tendency in the free silica group more markedly than in the natural coal dust group. The exud떼 te in alveolar space at 3rd week was disappeared at 20th week, and pneumoconiotic nodules observed microscopically in all experimental groups at 20th week , while the nodules apeared already at 3rd week in the 30 mg and 50 mg free silica dust groups. The significant increase of Hydroxyproline content in lung tissue and pneumoconiotic nodule formation in experimental groups found in this study indicate that the observation period, dust amount and kind of dust is important factors associated with pneumoconiosis. And these findings were generally more severe in free silica dust groups than in natural coal groups.
Key Words: Hydroxyproline, experimental pneumoconiosis, Free silica, Natural coal, Pathologic finding
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