| The Effects of Air-borne Particulate Matters on the Alveolar Macrophages for the TNF-alpha and IL-1beta Secretion. |
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Tian Zhu Li, Soo Jin Lee, Se Jong Park, Byung Joon Chang, Jong Hwan Lee, Kil Soo Kim, Myoung Heon Lee, Nong Hoon Choe |
1Konkuk University College of Veterinary Medicine, Seoul, Korea. nojamaji@hanmail.net
2Kyungpook National University College of Veterinary Medicine, Korea.
3National Veterinary Research and Quarantine Service, Korea. |
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| Abstract |
BACKGROUND
PM is known to induce various pulmonary diseases, including asthma, cancer, fibrosis and chronic bronchitis. Despite the epidemiological evidence the pathogenesis of PM-related pulmonary diseases is unclear. METHODS: This study examined the effects of PM exposure on the secretion of TNF-alpha and IL-1beta in the cultured alveolar macrophages. The cultured primary alveolar macrophages were treated with the medium, PM (5~20 microgram/cm2), LPS (5ng/ml), and PM with LPS for 24h and 48h respectively. ELISA was used to assay the secreted TNF-alpha and IL-beta in the culture medium. Western blotting was used to identify and determine the level of proteins isolated from the culture cells. The cells cultured in the Lab-Tek(R) chamber slides were stained with immunocytochemical stains. RESULTS: PM induced TNF-alpha and IL-1beta secretion in the culturing alveolar macrophages, collected from the SPF and inflammatory rats. However, the effects were only dose-dependent in the inflammatory macrophages. When the cells were co-treated with PM and LPS, there was a significant synergistic effect compared with the LPS in the both cell types. CONCLUSION: PM might be play an important role in the induction and/or potentiation of various lung diseases by oversecretion of TNF-alpha and IL-1beta. |
| Key Words:
Particulate matter (PM), Alveolar macrophages, Tumor necrosis factor-alpha (TNF-alpha), Interleukin 1beta (IL-1beta) |
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