The Comparison of the Effect of Cigarette and Stop Smoking-aiding Cigarette on Release of IL-6 from Bronchial Epithelial Cell.

Kim, Myoung Chan; Jung, Jeil; Jung, Jong Hoon; Kim, Hak Ryul; Yang, Sei Hoon; Jeong, Eun Taik; Kim, Hui Jung

doi:2005.59.5.530

Tuberc Respir Dis > Volume 59(5); 2005 > Article

Original Article

Tuberculosis and Respiratory Diseases 2005;59(5):530-535.
DOI: https://doi.org/10.4046/trd.2005.59.5.530 Published online November 1, 2005.

The Comparison of the Effect of Cigarette and Stop Smoking-aiding Cigarette on Release of IL-6 from Bronchial Epithelial Cell.

Myoung Chan Kim, Jeil Jung, Jong Hoon Jung, Hak Ryul Kim, Sei Hoon Yang, Eun Taik Jeong, Hui Jung Kim

¹Department of Internal Medicine, Seoul Adventist Hospital, Korea.
²Department of Internal Medicine, WonKwang University , School of Medicine, Sanbon Medical center, KunPo Korea. hikim61@hotmail.com

Abstract

BACKGROUND
AND AIMS: Cigarette smoking induces an inflammatory response in the airways, which may play a key role in the pathogenesis of chronic obstructive pulmonary disease. Interleukin-6 (IL-6) is one of the cytokines that plays an important role in inducing bronchial inflammation. The aim of this study was to determine if the level of the pro-inflammatory cytokine, Interleukin-6 , is increased when the bronchial epithelial cells are exposed to a cigarette smoke extract (CSE) and an extract from stop smoking-aiding cigarettes, and examined the safety of these commercially available stop smoking-aiding cigarettes. METHOD: Bronchial epithelial cells were exposed to CSE from cigarette and stop smoking-aiding cigarettes for 24 hours. ELISA was used to measure the IL-6 levels in the supernatant from each condition. The IL-6 mRNA levels were measured by Taqman Real time RT-PCR. N-acetyl-L-cysteine(NAC) was added to each condition to determine if NAC can inhibit the release of IL-6 from the bronchial epithelial cells when they are exposed to CSE from cigarette and stop smoking-aiding cigarettes. RESULT: When bronchial epithelial cells were exposed to a CSE from cigarettes and stop smoking- aiding cigarettes, each type of CSE stimulated IL-6 production from the bronchial epithelial cells. The IL-6 mRNA level in the Bronchial epithelial cells was also elevated and NAC was found to inhibit the release of IL-6 from bronchial epithelial cells when they were exposed to the CSE from cigarettes and stop smoking-aiding cigarettes. CONCLUSION: Commercially available stop smoking-aiding cigarette can induce bronchial inflammation and can be harmful to smokers. Therefore, the safety of these cigarettes for smoking cessation should be evaluated.

Key Words: Cigarette, stop smoking-aiding cigarette, IL-6