| Expression of Matrix Metalloproteinases-9 and Stromelysin-3 in Peripheral Blood in Patients with Lung Cancer. |
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Seong Yong Lim, Won Jung Koh, Cheol Hong Kim, Young Mee Ahn, Young Mee Kwon, Kyeong Woo Kang, Ho Cheol Kim, Gee Young Suh, Man Pyo Chung, Si Young Lim, Ho Joong Kim, O Jung Kwon |
1Division of Pulmonary and Critical Care Medicine, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea. hjkim@smc.samsung.co.kr
2Samsung Biomedical Research Institute, Seoul, Korea.
3Division of Pulmonary and Critical Care Medicine, Department of Medicine, Masan Samsung Hospital, Masan, Korea.
4Division of Pulmonary and Critical Care Medicine, Department of Medicine, Kangbuk Samsung Hospital, Seoul, Korea. |
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| Abstract |
BACKGROUND
Matrix metalloproteinases(MMP) are essential enzymes for tumor invasion and metastasis. Among the MMP family, elevated MMP-9 and stromelysin-3(STR-3) expression have been reported to be poor prognostic factors in lung cancer patients. To evaluate the possibility of a molecular diagnosis of lung cancer using peripheral blood, the mRNA expression level of MMP-9 and STR-3 was measured using a reverse transcriptase-polymerase chain reaction(RT-PCR) in patients with lung cancer. METHODS: Ninety six patients(44 patients with lung cancer, 19 pulmonary infection, and 33 control) were included. To detect MMP-9 and STR-3 mRNA expression, RT-PCR was performed in peripheral blood mononuclear cells. ELISA was also used to measure the serum level of MMP-9. RESULTS: MMP-9 was expressed more frequently in patients with a pulmonary infection(18/19, 94.7%) compared to lung cancer patients(26/44, 59.1%) or the controls (23/33, 69.7%) (p=0.018). On the other hand, STR-3 expression was observed more frequently in patients with lung cancer(37/44, 84.1%) compared to the lung infection patients(8/19, 42.1%) or control(20/33, 60.6%) (p=0.003). Among the lung cancer patients, MMP-9 was expressed more frequently when a tumor invaded the lymph nodes(17/24, 70.8%) compared to when a tumor did not(3/13, 23.1%) (p=0.005). The MMP-9 and STR-3 expression levels had no relationship with age, sex, tumor size, distant metastasis, or tumor histology. The serum MMP-9 concentration was not higher in lung cancer patients compared to patients with a pulmonary infection or the control subjects. CONCLUSION: STR-3 may be used as a diagnostic marker in the peripheral blood of lung cancer patients using RT-PCR. Further studies to evaluate the clinical significance of elevated STR-3 expression in lung cancer patients is recommended. |
| Key Words:
Matrix metalloproteinases, Bronchogenic carcinoma, Reverse transcriptase plolymerase chain reaction |
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