Tuberc Respir Dis > Volume 51(4); 2001 > Article
Tuberculosis and Respiratory Diseases 2001;51(4):303-314.
DOI: https://doi.org/10.4046/trd.2001.51.4.303    Published online October 1, 2001.
Matrix Metalloproteinase in Idiopathic Pulmonary Fibrosis.
Joohun Park, Tae Sun Shim, Chae Man Lim, Younsuck Koh, Sang Do Lee, Woo Sung Kim, Won Dong Kim, Dong Soon Kim
Abstract
BACKGROUND
Matrix metalloproteinase(MMP)-2 and MMP-9 have been known to play an important role in cell migration and the tissue remodeling process by type IV collagen lysis, a major component of the basement membrane. Intra-alveolar fibrosis, secondary to an injury to the basement membrane of the alveolar epithelial lining, is a major process in the pathogenesis of idiopathic pulmonary fibrosis(IPF). Therefore, MMP-2 and MMP-9 was hypothesized to play an important role in IPF pathogenesis. As a result, their level may reflect the activity or prognosis. METHOD: Forty one progressive IPF patients(age 59.82±1.73 years, M:F=23:18), 16 patients with stable IPF for more than one year without therapy(age: 63.6±2.8 years, M:F=13:3), and 7 normal controls were enrolled in this study. The MMP-2 and MMP-9 levels in the BAL fluid and alveolar macrophage conditioned media(AM-CM) were measured by zymography ans the TIMP-1 level was measured by ELISA. RESULTS: 1) The MMP-2 level in BALF was highest in the progressive IPF group (1.36±0.28) followed by the stable group (0.46±0.13) and the controls (0.08±0.09). which was statistically significant. The MMP-9 level of the IPF (0.31±0.058) and the stable group (0.22±0.078) were higher than that of the control group (0.002±0.004). In the AM-CM, only MMP-9 was detected, which was significantly higher in IPF group (0.80±0.10) than in the control group (0.23±0.081) The MMP-1 level was also higher in both the IPF (36.34±8.62 µg/ml) and stable group (20.83±8.53 µg/ml) compared to the control group (2.80±1.05 µg/ml) (p<0.05). 3) There was a correlation between the MMP-2 level in the BALF with the total cell number(r=0.298) and neutrophils(r=0.357) (p<0.05), and the MMP-9 level with the number of neutrophils (r=0.407) and lymphocytes (r=0.574) (p<0.05). The TIMP-1 level correlated with the total number of cell (r=0.338, p<0.05) and neutrophils (r=0.449, p=0.059). CONCLUSION: Both MMP and TIMP appear to play an important role in IPF pathogenesis, and their level may reflect the disease activity.
Key Words: Idiopathic Pulmonary Fibrosis, Matrix Metalloproteinase, Tissue Inhibitor of Matrix Metalloproteinase, BAL, Prognosis


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