| Evaluation of the Cell-Mediated Immunity in Treatment Failure Pulmonary Tuberculosis. |
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Jeong Kyu Park, Jang Seo Park, Hwa Jung Kim, Eun Gyeong Jo, Dullei Min, Jae Hyun Lim, Ji Won Suhr, Tae Hyun Paik |
1Department of Microbiology, College of Medicine, Chungnam National University, Taejon, Korea.
2Department of Internal Medicine, Catholic University, Taejon, Korea.
3Department of Microbiology, College of Medicine, Konyang University, Nonsan, Chungnam, Korea. |
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| Abstract |
BACKGROUND
Ineffective cell-mediated immune response in human tuberculosis is associated with a depressed Th1 cytokine response and reduced production of IFN-gamma. Most persons infected with Mycobacterium tuberculosis are healthy tuberculin reactors with protective immunity, but a minority with ineffective immunity develop extensive pulmonary tuberculosis. The cell-mediated immune response is an important aspect of host resistance to mycobacterial infection and is believed to be tightly regulated by a balance between Th1 cytokines including IFN-gamma IL-12, IL-18, regulated on activation, normal T cell expressed and secreted (RANTES) and Th2 counterparts such as IL-4, monocyte chemoattractant protein-1 (MCP-1). METHODS: Proliferation and mRNA expression of IFN-gamma RANTES and MCP-1 by RT-PCR in peripheral blood mononuclear cells (PBMCs) in response to in vitro stimulation with mycobacterial antigens were compared in pulmonary tuberculosis patients with cured and treatment failure and in tuberculin-positive and tuberculin-negative healthy subjects. RESULTS: Defective proliferative responsiveness to aqueous TSP antigen was involved with treatment failure tuberculosis patients. Aqueous TSP antigen-induced IFN-gamma and RANTES mRNA expression was decreased in treatment failure tuberculosis patients compared with healthy tuberculin reactors and cured tuberculosis patients (23.1% versus 90.0% for IFN-gamma and 46.2% versus 70.0% versus 46.2% for RANTES). The frequency of MCP-1 mRNA expression to aqueous TSP antigen in treatment failure tuberculosis patients was greater than in healthy tuberculin reactors and cured tuberculosis patients (76.9% versus 40.0%). CONCLUSION: The increasing expression of MCP-1 mRNA in response to aqueous TSP antigen might be predicted to favor Th2 responses and restricted Th1 responses in treatment failure of pulmonary tuberculosis. |
| Key Words:
Pulmonary tuberculosis, IFN-gamma, Monocyte chemoattractant protein-1 |
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