| Expression of Transforming Growth Factor-alpha and Transforming Growth Factor-beta In Human Primary Lung Cancers. |
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Woo Jin Lew, Dong Ho Shin, Sung Soo Park, Dong Hoo Lee, Jung Dal Lee, Jung Hee Lee |
1Department of Internal Medicine, Hanyang University, College of Medicine, Seoul, Korea.
2Department of Pathology, Hanyang University, College of Medicine, Seoul, Korea. |
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| Abstract |
BACKGROUND
Transforming growth factor- alpha(TGF-alpha) may play important roles in carcinogenesis, tumor growth, and angiogenesis. Transforming growth factor-beta(TGF-beta) are known to be involved in cell-cycle control and regeneration. TGF-alpha positively acts on growth control of many epithelial cells in contrast to the negative role of TGF-beta. METHOD: To evaluate the possible role of TGF-alpha and TGF-beta in human primary lung cancers, the expression of TGF-alpha and TGF-beta were immmunohistochemically investigated in tissue sections from forty seven cases with lung cancers and ten cases with non-cancerous lung tissues. Recombinant cloned monoclonal antibody of TGF-alpha and neutralizing antibody of TGF-beta were employed as primary antibodies after dewaxing the formalin-fixed, paraffinized tissue sections. RESULTS: TGF-alpha was expressed in the cytoplasms of tumor cells in thirty five cases of forty seven(74.5%) primary lung cancers, whereas the control expressed in two of ten brochial epithelial cells. The expression of TGF-alpha was disclosed in four cases of eleven(36.4%) small cell carcinomas and thirty one cases of thirty six(86.1%) non-small cell carcinomas of the lung. Expressions of TGF-beta was discernible in bronchial epithelium in eight of ten non-cancerous lung tissues. The expression of TGF-beta was noted in the cytoplasms of tumor cells in eight cases of forty seven(17.0%) primary lung cancers. The expression of TGF-beta disclosed in two cases of eleven(18.2%) small cell carcinomas and six cases of thirty six(16.7%) non- small cell carcinomas of the lung. CONCLUSION: These findings suggest that up-regulation of TGF-alpha and down-regulation of TGF-beta are involved during development and growth of primary lung cancers. |
| Key Words:
Expression, Transforming growth factor alpha and beta, Lung cancer |
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