Loss of the retinoblastoma gene in non-small cell lung cancer. |
Choon Taek Lee1, Chang Min Kim1, Jae Ill Zo2, Young Mag Shim2, Weon Seon Hong1, Jhin Oh Lee1, Tae Woong Kang1 |
1Department of Internal Medicine, Korea Cancer Center Hospital, Seoul, Korea 2Department of Thoracic Surgery, Korea Cancer Center Hospital, Seoul, Korea |
비소세포폐암에서의 망막모세포종유전자의 소설 |
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Abstract |
Background 1nactivation of retinoblastoma gene (Rb) has been observed in a variety of human cancers. Loss of heterozygosity (LOH) of Rb which is a common mode of allelic inactivation of Rb, has been known as a frequent genetic event in small cell lung cancer but it has been detected less frequently in non-small cell lung cancer. To define the role of Rb deletion in lung cancer, we investigated the genomic DNAs of 43 non-small cell lung cancers and 1 small cell lung cancer paired with normal lung tissues obtained by thoracotomy.
Methods The genomic DNAs were obtained by the digestion with proteinase K followed by phenol-chloroform extraction method. The genomic DN As were digested by restriction endonuclease (EcoRI), separated by agarose gel electrophoresis, transferred to nylon membrane by Southern blot transfer and then hybridized with labelled Rb 1 probe which contains. 1. 4 kb sized DNA sequence containing N-terminal portion of Rb.
Results 1n 26 squamous cell lung cancers, 16 cases were informative after EcoR1 digestion and LOH of Rb was found in 10 cases (62.5%). 1n 17 adenocarcinomas of lung, 11 cases were informative and LOH of Rb was found in five cases (45.4%). The analysis of clinical parameters revealed no significant differences between the two groups with or without LOH of Rb in the aspects of age, sex, degree of differentiation, stage and smoking amount.
Conclusions These results suggest that Rb inacti vation is also significantly involved in the molecular pathogenesis of non-small cell lung cancer |
Key Words:
Rb, Non-small cell lung cancer, LOH, Southern blot |
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