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Tuberc Respir Dis > Volume 40(2); 1993 > Article
Original Article
Tuberculosis and Respiratory Diseases 1993;40(2):98-103.
DOI: https://doi.org/10.4046/trd.1993.40.2.98    Published online April 1, 1993.
Loss of the retinoblastoma gene in non-small cell lung cancer.
Choon Taek Lee1, Chang Min Kim1, Jae Ill Zo2, Young Mag Shim2, Weon Seon Hong1, Jhin Oh Lee1, Tae Woong Kang1
1Department of Internal Medicine, Korea Cancer Center Hospital, Seoul, Korea
2Department of Thoracic Surgery, Korea Cancer Center Hospital, Seoul, Korea
비소세포폐암에서의 망막모세포종유전자의 소설
Abstract
Background
1nactivation of retinoblastoma gene (Rb) has been observed in a variety of human cancers. Loss of heterozygosity (LOH) of Rb which is a common mode of allelic inactivation of Rb, has been known as a frequent genetic event in small cell lung cancer but it has been detected less frequently in non-small cell lung cancer. To define the role of Rb deletion in lung cancer, we investigated the genomic DNAs of 43 non-small cell lung cancers and 1 small cell lung cancer paired with normal lung tissues obtained by thoracotomy.
Methods
The genomic DNAs were obtained by the digestion with proteinase K followed by phenol-chloroform extraction method. The genomic DN As were digested by restriction endonuclease (EcoRI), separated by agarose gel electrophoresis, transferred to nylon membrane by Southern blot transfer and then hybridized with labelled Rb 1 probe which contains. 1. 4 kb sized DNA sequence containing N-terminal portion of Rb.
Results
1n 26 squamous cell lung cancers, 16 cases were informative after EcoR1 digestion and LOH of Rb was found in 10 cases (62.5%). 1n 17 adenocarcinomas of lung, 11 cases were informative and LOH of Rb was found in five cases (45.4%). The analysis of clinical parameters revealed no significant differences between the two groups with or without LOH of Rb in the aspects of age, sex, degree of differentiation, stage and smoking amount.
Conclusions
These results suggest that Rb inacti vation is also significantly involved in the molecular pathogenesis of non-small cell lung cancer
Key Words: Rb, Non-small cell lung cancer, LOH, Southern blot
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