Introduction
Chronic obstructive pulmonary disease (COPD) is a common, preventable and treatable disease that is characterized by persistent respiratory symptoms and airflow limitation that is due to airway and/or alveolar abnormalities usually caused by significant exposure to noxious particles or gases [
1]. COPD is a leading cause of morbidity and mortality worldwide that is associated with significant economic and social burden, which are both substantial and increasing. Tobacco smoking is the most important causative factor, but the marked variability in lung function in people with similar cigarette smoking histories suggests an important role for genetics and other factors in COPD.
Lungs from patients with COPD are characterized by inflammatory cellular infiltration and remodeling of small airways with thickening of their walls and narrowing of their lumen. The histological features associated with these changes include inflammatory cell infiltration of the alveolar walls, as well as destruction and enlargement of air spaces beyond the terminal bronchioles. The mixture of small airway obstruction and parenchymal destruction of emphysema contributes to airflow limitation in COPD [
2].
It is well accepted that lung inflammation plays an important role in the pathogenesis of COPD, but the type of inflammatory cells that participate in this destruction remains unclear. It was reported that smokers who develop chronic airflow limitation have an increased number of CD8
+ T lymphocytes in the peripheral airways [
3], supporting the important role of CD8
+ T lymphocyte in the pathogenesis of COPD. An increase in apoptosis of airway epithelial cell has also been suggested to contribute to COPD pathogenesis [
4]. The role of granzyme B (GzmB) was proposed [
5], because CD8
+ T cells are able to induce apoptosis of target cells by releasing GzmB and non-specifically released GzmB may accumulate in the extracellular milieu, leading to tissue destruction and parenchymal remodeling [
6]. Indeed, GzmB levels as well as T cells expressing GzmB are increased in bronchoalveolar lavage (BAL) fluid of COPD patients [
7]. However, to our knowledge, the localization of GzmB
+ cells on the alveolar wall has not been reported in emphysema. On the other hand, GzmB has been shown to be expressed in not only CD8
+ T cells, but also in many other cells such as regulatory cells [
8], which are able to suppress CD8
+ T cells.
The role of alveolar GzmB+ cells in centrilobular emphysema, which is an important component of COPD is largely unknown. In this study, we explored the role of GzmB+ cells in the process of lung destruction by determining the number of alveolar GzmB+ cells according to the degree of airflow limitation in anatomically documented centrilobular emphysematous lungs.
Discussion
In the present study we demonstrated that the number of GzmB
+ cells on the alveolar wall of subjects with CLE is directly related to FEV
1. We also showed that alveolar GzmB
+ and CD8
+ cells are present in greater numbers in CLE compared with those in control or PLE lungs. Notably, on dual staining for GzmB and CD8, not all GzmB
+ cells were CD8 positive and not all CD8
+ cells were GzmB positive (
Figure 1C,
D).
COPD is not a single disease entity and pathologically it consists of emphysema and small airway disease. CLE is the most common subtype of emphysema in COPD [
11,
12] and this type of emphysema is associated with small airway disease [
9,
13,
14].
A few morphologic studies [
15-
18] have attempted to directly describe the nature of inflammatory cells on the alveolar wall of emphysema, where actual lung destruction occurs. Increased number of CD8
+ cells on alveolar wall was reported in emphysema, but the numbers of natural killer cell, which is another source of GzmB, were not increased in this study [
17]. Apoptosis induced by cytolytic function of CD8
+ T cell was suggested as one of the mechanisms for lung destruction.
The role of autoimmunity in the pathogenesis of COPD is increasingly recognized [
19]. It was suggested that CD8
+ T cells are recruited upon tobacco-modified self-antigenic stimulation and activated to become GzmB
+ cells in the pathogenesis of COPD. The present result demonstrating increased number of CD8
+ T cells in CLE suggests involvement of these cells in lung destruction. However, localization of GzmB
+ cell, which is a marker of activated cytolytic CD8
+ T cell, on the alveolar wall of emphysema has not been reported and actual role of GzmB
+ cell in COPD is not known.
The present study showed a higher number of alveolar GzmB
+ cells in mild emphysema and a lower number of GzmB
+ cells in severe one. This trend was also present in volume fraction of GzmB
+ cells in the small airway wall of CLE subjects [
10]. In addition, lung tissue expression of GzmB was also positively correlated with FEV
1 in smokers [
10]. With this result we were not certain whether GzmB
+ cells are effector of cytolysis in the pathogenesis of emphysema or controllers over the effector CD8
+ T cells that cause tissue damage in these emphysema subjects.
In a previous report [
10], we found that cases with mild airflow limitation may represent early stages of CLE, therefore they demonstrated increased volume fraction of GzmB
+ cells for active cytolytic function in the small airway wall. However, in order to explore the role of GzmB
+ cells as an effector of cytolysis, identification of GzmB
+ CD8
+ T cell in early stages of lung destruction would be necessary. To our knowledge, no such study has been reported in early COPD subjects.
It was shown that only recently activated CD8
+ T cells are capable of GzmB production [
20]. In chronic infections and cancer, T cells are exposed to persistent antigen and/or inflammatory signals and T cell is exhausted to manifest progressive loss of effector functions [
21]. This fact suggests that GzmB containing cells in “old” COPD subjects may not necessarily be CD8
+ T cells. Consistent with this notion, we showed that not all GzmB
+ cells were CD8 positive and not all CD8
+ cells were GzmB positive on dual staining. Additionally, the previously reported result showed that volume fraction of GzmB
+ cells (2.70±1.08%) was greater than that of CD8
+ cells (2.02±0.56%) in the small airway wall of the same CLE subjects (p=0.006) [
10].
Regulatory T (Treg) cells play a crucial role in maintaining peripheral tolerance and preventing autoimmunity. It was reported that COPD patients had fewer BAL fluid Treg cells than healthy smokers and it was hypothesized that an insufficient number of regulatory T cells to CD8
+ T cell ratio may contribute to COPD [
22]. Significantly fewer Treg cells were present in the resected lungs of subjects with emphysema relative to control subjects [
23]. COPD subjects showed decreased Treg cells in BAL fluid and they displayed positive correlations with FEV
1 [
24]. Immunohistochemical study of lung tissue of resected lungs also showed decreased number of Treg cells in the small airways of COPD and they positively correlated with FEV
1 [
25].
GzmB was not expressed in naïve Treg cells, but was highly expressed in Treg cells in the tumor environment in animal study [
26]. It was found that tumor cells induced GzmB expression in Treg cells, which in turn utilized GzmB to suppress tumor clearance mediated by CD8
+ T cells. In human endometrial carcinoma, Treg cells in the tumor microenvironment can express significantly higher levels of GzmB. GzmB was rarely expressed by CD8
+ T cell-derived tumor-infiltrating lymphocytes [
27]. One of mechanisms by which Treg cells control the immune response is by inhibiting cytolysis of effector cell through the production of granzymes [
28]. In an animal study, depletion of Treg cells before respiratory syncytial virus challenge led to a marked increase in the influx of inflammatory cells into the lung, and boosting Treg numbers reduced pathology. The ability of Treg cells to regulate lung inflammation depended on their ability to produce GzmB [
8]. In addition to Treg cells, human plasmacytoid dendritic cell (pDC) can be an abundant source of GzmB and GzmB
+ pDC potentially suppresses T-cell proliferation in a GzmB-dependent manner [
29]. Also it was reported that the microenvironment of various solid tumors includes B cells that express GzmB, which suppress T-cell proliferation by GzmB-dependent degradation of the T-cell receptor [
30]. Because Treg cell, pDC and B cell are known to modulate T effector cells by a GzmB-dependant mechanism, it was hypothesized that GzmB may be a common effector molecule of human regulatory cells in general [
30]. Together, these findings raise the possibility that most of alveolar GzmB
+ cells in the present older emphysema subjects could be GzmB expressing regulatory cells.
The finding of higher numbers of alveolar GzmB+ cells in older CLE subjects with relatively preserved FEV1 in the present study suggests that greater GzmB expressing regulatory cells might have suppressed more effector T cells, resulting in less lung destruction and relative preservation of lung function in these CLE subjects.
GzmB is a common mediator for CD8
+ effector T cell and regulatory cells which suppress effector T cell; therefore, GzmB may play a dual opposing role in target cell destruction. It was reported that in immune surveillance, GzmB, the most potent cytotoxic molecules, act mainly as an antitumoral and anti-infectious factor, but when expressed by immune regulatory cells they may contribute to immune evasion of specific cancer types [
31].
Although GzmB
+ CD8
+ T cell is presumed to have a role as an effector cell in early stages of COPD, it remains a mystery as to how very low GzmB in CD8
+ effector T cell can exert a cytolytic function. It is known that CD8
+ T lymphocytes express both GzmB and granzyme A (GzmA), but Treg cells induce expression of GzmB only [
32]. Although there are several mechanisms by which CD8
+ effector T cells exert cytotoxic action, there was a report that CD8
+ T cells lacking GzmB retain cytotoxic activity through the action of the GzmA in an animal study [
33]. Also it was reported that in the lungs of very severe COPD subjects who underwent lung transplantation, bronchiolar epithelium stained positive for GzmA, but not for GzmB and intense staining for GzmA in type II pneumocytes [
34]. This extracellularly released GzmA suggests the finding that GzmA pathway is activated and GzmA is resultantly leaked out. Thus the low alveolar GzmB
+ cells in severe CLE observed in the present study might have played reduced suppressive action on effector CD8
+ T cells, which in turn may have enhanced cytolytic effector T cell function through GzmA pathway, resulting in increased lung damage. However, it is not known what causes low GzmB in severe COPD. One possibility is that GzmB related genetic influence modulates GzmB at the cellular level, but further study is needed to validate the reason.
We posit that the role of alveolar CD8
+ T and GzmB
+ cells in the pathogenesis and progression of emphysema is different in CLE compared with PLE. We observed an increase in alveolar CD8
+ T and GzmB
+ cells in CLE (a tobacco-related emphysema) compared with control or PLE lungs. Although naïve, quiescent T cells cannot egress directly from blood into lung parenchyma, activated T cells by antigen-bearing dendritic cells can be drawn into lung tissue [
19]. It should be noted that cytolytic T cell and Treg cell activation is antigenspecific. Thus, we speculate that these cells play an important role in the pathogenesis of CLE but not PLE. In contrast to CLE, whose emphysematous lesions are small and discrete, PLE lungs show diffuse emphysematous changes throughout the secondary lobules. PLE may be driven by an imbalance in the proteolytic-antiproteolytic enzymatic activity in the alveolar units and thus may be less dependent on T cell biology or cell-mediated pathology. Therefore CLE and PLE are likely different diseases even though clinically they ultimately lead to airflow limitation. Future research study on COPD should be done according to the subtypes of emphysema. This study showed that some mild emphysema cases can have no airflow limitation even though they have definite anatomic emphysema on microscopic examination. Also it is notable that GzmB
+ cell might have common associated role in the progression of both emphysema and small airway disease in CLE.
There are several limitations to the study. We included both Asian and Caucasian subjects, which might have introduced confounding by ethnicity, but there were no differences in the number of alveolar GzmB
+ and CD8
+ cells between the two races in control and PLE subjects. The recruited number of study subjects was not large enough to validate the results, but it was due to innate complexity of morphometric study of human lungs to be performed. Another limitation was that this study was cross-sectional. Further, although the age distribution of mild and severe emphysema subjects was not significantly different, we did not have accurate information on the duration or intensity of smoking for our cohort. The mean age of cases with FEV
1 below 80% predicted (n=11, FEV
1 52.7±18.2%) was 62.5±6.0 years and that of cases with FEV
1 80% or above (n=19, FEV
1 100.6±12.9%) was 62.9±8.8 years. Given a similar age distribution, we postulate that the differences in the severity of airflow limitation represent differences in individual’s genetic susceptibility to COPD as proposed by a previous report [
19]. We did not have quantitative data for dual staining of GzmB
+ and CD8
+ cells because our primary aim was to determine the number of alveolar GzmB
+ and CD8
+ cells according to the severity of airflow limitation in CLE. Also we did not have data on dual staining for Treg and GzmB, because it was beyond the purview of the present study. Additional studies will be required to quantitate these cells in lungs of COPD patients.
In conclusion, this study shows that GzmB+ and CD8+ cells are upregulated on the alveolar wall of CLE compared to control or PLE lungs. There is a strong positive relationship between the number of alveolar GzmB+ cells and FEV1 in CLE subjects. These data suggest that GzmB+ cell may play an important role in determining the extent of lung destruction and degree of airflow limitation in centrilobular type of emphysema.