A Study on Newly Formulated Mycobacterial Media and Their Application in Rapid Diagnosis of Chronic Mycobacterial Diseases

Lew, Joon; Lee, Young Nam; Kim, Sung Kwang

doi:1978.25.2.57

Tuberc Respir Dis > Volume 25(2); 1978 > Article

Original Article

Tuberculosis and Respiratory Diseases 1978;25(2):57-62.
DOI: https://doi.org/10.4046/trd.1978.25.2.57 Published online June 1, 1978.

A Study on Newly Formulated Mycobacterial Media and Their Application in Rapid Diagnosis of Chronic Mycobacterial Diseases

Joon Lew, Young Nam Lee, Sung Kwang Kim

Dept. of Microbiology, Yonsei Univ., College of Medicine, Seoul 120, Korea

항산균에 의한 만성감염 질환의 세균학적 조기진단 및 배양법 개량에 관한 연구

유준, 이영남, 김성광

Abstract

We made attempts to formulate simple media supporting better growth of mycobacterial species and to evaluate feasibility of the media using in rapid diagnosis of the chronic mycobacterial diseases. One of the media formulated is a phosphate buffered medium with 0.1 % hyaluronic acid and 6.0% bovine serum albumin in addition to 3.0% glycerol. This medium was originally formulated by Skinsnes’ group, but we made slight modification. The other is a semisynthetic medium made of umbilical cord extract supplemented with 10% sheep serum as a final concentration. Mycobacterial species employed in the experiments gave better growth yield in the newly formulated media than the conventional Dubos liquid medium. We also checked pathogenicity of M. tuberculosis H₃₇Rv grown in the different media to mouse. Tubercle bacilli grow in the Dubos liquid medium showed more potent pathogenicity to mouse than those grown in the newly formulated media.