Tuberc Respir Dis > Volume 25(3); 1978 > Article
Tuberculosis and Respiratory Diseases 1978;25(3):115-119.
DOI: https://doi.org/10.4046/trd.1978.25.3.115    Published online September 1, 1978.
Interaction between Mycobacterium Fortuitum and Macrophages from the Mouse Peritoneal Exudate -Concerning Intracellular Ecology-
Chang Yong Cha
Department of Microbiology, College of Medicine, Seoul National Uniνersity, Seoul, Korea
Mycobacterium Fortuitum의 마우스 거식 세포에 대한 반응양상 -세포내 생태에 관하여-
차창룡
Abstract
Numerous studies on the interaction between phagocytes and pathogen were reported; however, informations on the infection-establishing mechanisms by M. fortuitum is incomplete. It would be worthwhile to learn mechanisms involved M. fortuitum infections as a consequence of the relationship between host and parasite. Thus, an experiment was performed to study the mode of interactions between mouse peritoneal macrophages and M. fortuitum in vitro condition. Normal mouse peritoneal exudate collected, was pooled, suspended in the cellmaintenance medium and mixed with the suspension of M. Fortuitum. The bacteriamacrophages suspension in the Leighton tube with cover-slip were incubated at 37℃. for 120 minutes. After 30 minutes' interval of incubation period the ratios of the macrophages associated with bacteria to the total ones and the distribution of the macrophages laden with bacteria by the bacterial number were measured from the stained cover-slip. The resutls obtained were summerized as follows: 1. Ratios of macrophages associated or containing M. fortuitum rapidly increased to 52% until 90 minutes' incubation and thereafter, graually increased to 57.8% at 120 minutes. 2. During incubation period, continuation of increase in the number of macrophage contaning many baccili resulted in increase of total number of M. fortuitum.
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