Immunocytochemical Study on the Change of the Percentage and Absolute Numbers of T Lymphocytes and their Subsets in the Peripheral Blood and Pleural Effusion of Tuberculosis Patients

Kim, Joon Woo; Shim, Young Soo; Kim, Keun Youl; Han, Yong Chol; Lee, Wang Jae; Chang, Ka Young

doi:1987.34.3.197

Tuberc Respir Dis > Volume 34(3); 1987 > Article

Original Article

Tuberculosis and Respiratory Diseases 1987;34(3):197-208.
DOI: https://doi.org/10.4046/trd.1987.34.3.197 Published online September 1, 1987.

Immunocytochemical Study on the Change of the Percentage and Absolute Numbers of T Lymphocytes and their Subsets in the Peripheral Blood and Pleural Effusion of Tuberculosis Patients

Joon Woo Kim¹, Young Soo Shim¹, Keun Youl Kim¹, Yong Chol Han¹, Wang Jae Lee², Ka Young Chang²

¹Department of Internal Medicine, College of Medicine, Seoul National University, Seoul, Korea
²Deparfmenf of Anatomy, College of Medicine, Seoul National University, Seoul, Korea

한국인 결핵환자의 말초혈액 및 늑막삼출액에서의 T림프구 및 그 아형들의 수적변화에 관한 면역세포화학적 연구

김준우¹, 심영수¹, 김건열¹, 한용철¹, 이왕재², 장가용²

Abstract

The immunocytochemical study was performed to observe the changes of the percentage and absolute numbers of T-Iymphocytes and their subsets in the peripheral blood and pleural effusion of tuberculosis patients The peripheral blood obtained from fifteen healty persons and thirty-eight pulmonary tuberculosis patients (minimal 14, moderate 11, far advanced 13), and the pleural fluid from tuberculous pleruisy patients were smeared onto the gelatin coated slide glass prepared for the smear of mononuclear cells. The double bridge technique of alkaline phosphatase-antialkaline phosphatase (AP AAP) method was lIsed. As the primary antibodies, T₁ (anti-human T cells), T₄ (anti-human helper/ inducer T cells) and T ₉ (anti-human supressor/ cytotoxic T cell) antibodies were used. The following results were obtained from this experiment. 1) The percentage of T _{1 +} and T _{4 +} cells showed marked decrease in the pulmonary tuberculosis and tuberculous pleurisy patients. That of T₈₊ cells showed no change. T ₄₊ / T₈₊ ratio showed marked decrease in all experimental groups. 2) There are no differences in the percentage of T_{4 +} cells and T_{4 +}/ T₈₊ ratio between each group of Pulmonary tuberculosis patients. 3) The tuberculous pleurisy patients showed decrease in the percentage of T_{4 +} cells but no change in that of T₈₊ cells in the peripheral blood, and showed 76% of T_{1 +} cells, 68% of T_{4 +} cells and 17% of T₈₊ cells in the pleural effusion. 4) There were significant decreases in the absolute number of T_{1 +} , T_{4 +} and T₈₊ cells in the peripheral blood of all groups of tuberculous patients. 5) There were positive correlations between the long diameter of induration caused by tuberculin skin test and the percentage of T_{1 +}cells, T_{4 +} cells and T_{4 +}/T ₈₊ ratio.