| Relations between Polymorphism of NRAMP1 Gene and Susceptibility to Pulmonary Tuberculosis. |
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Ji Seok Lee, Ki Uk Kim, Hye Kyung Park, Yun Seong Kim, Ho Seok Lee, Young Dae Kim, Doo Soo Jeon, Seung Kyu Park, Min Ki Lee, Soon Kew Park |
1Department of Internal Medicine, College of Medicine, Pusan National University, Busan, Korea. leemk@pusan.ac.kr
2Department of Thoracic Surgery, College of Medicine, Pusan National University, Busan, Korea.
3Masan National Hospital, Masan, Korea. |
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| Abstract |
Background
Several lines of evidence suggest that a host's genetic factors influence the outcome of exposure to Mycobacterium tuberculosis. The aim of this study was to determine whether polymorphism in NRAMP1 (natural resistance associated macrophage protein 1) gene is associated with the susceptibility or resistance to tuberculosis infection for patients with drug-sensitive pulmonary tuberculosis (DS-TB) and multi-drug resistant pulmonary tuberculosis (MDR-TB). Methods: Eight genetic polymorphisms of the NRAMP1 gene were investigated in patients suffering with DS-TB (n=100) or MDR-TB (n=102), and in healthy normal controls (NC, n=96). The genetic polymorphisms of NRAMP1 were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results: The frequency of D543N A/G heterogygotes was significantly higher in the DS-TB subjects than the NCs (OR=2.10, 95% CI: 1.00 to 4.41, p=0.049). The frequency of 823C/T T/C heterozygotes was significantly higher in the DS-TB subjects (OR=2.79, 95% CI: 1.11 to 7.04, p=0.029) and the MDR-TB subject (OR=3.30, 95% CI 1.33 to 8.18, p=0.010) than in the NCs. However, the frequency of these genotypes was not different between the DS-TB and MDR-TB subjects. Conclusion: A significant association was found between NRAMP1 823 C/T polymorphism and pulmonary tuberculosis. This result suggests that NRAMP1 polymorphism may be involved in the development of pulmonary tuberculosis in Koreans. |
| Key Words:
NRAMP1, Polymorphism, Tuberculosis |
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